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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
17.9K
Multifunctional solvothermal carbon derived from alginate using 'water-in-deep eutectic solvents' for enhancing
Niketa Yadav1, Manohara Halanur M, Meena Bisht
1Department of Chemistry, University of Delhi, Delhi - 110 007, India. pvenkatesu@chemistry.du.ac.in.
Chemical Communications (Cambridge, England)
|July 23, 2020
Summary
Researchers developed a sustainable method using a water-in-deep eutectic solvent (DES) system to create alginate-derived carbon (AAC) materials. These AACs enhanced the activity and stability of immobilized cytochrome-c (Cyt-c) for biotechnological applications.
Area of Science:
- Materials Science
- Biotechnology
- Green Chemistry
Background:
- Alginic acid (AA) conversion into functional carbon materials is crucial for various applications.
- Developing sustainable and low-temperature synthesis methods is an ongoing challenge.
- Enzyme immobilization on carbon supports can enhance stability and activity.
Purpose of the Study:
- To develop a novel, low-temperature method for synthesizing alginate-derived carbon (AAC) materials using a water-in-deep eutectic solvent (DES) system.
- To investigate the immobilization of cytochrome-c (Cyt-c) onto the synthesized AAC materials.
- To evaluate the impact of AAC materials on the enzymatic activity and stability of Cyt-c.
Main Methods:
- Utilized a water-in-deep eutectic solvent (DES) system for the low-temperature conversion of alginic acid (AA) to alginate-derived carbon (AAC).
- Immobilized cytochrome-c (Cyt-c) onto the surface of the synthesized AAC materials, leveraging their inherent oxygen functionalities.
- Assessed the peroxidase activity of the immobilized Cyt-c and compared it to the native enzyme.
Main Results:
- Successfully synthesized multifunctional alginate-derived carbon (AAC) materials via a sustainable, low-temperature DES-based process.
- Achieved significant enhancement (up to 5.5-fold) in the peroxidase activity of immobilized Cyt-c compared to the native protein.
- Demonstrated that the AAC material preserved the structural stability of Cyt-c during immobilization and assay.
Conclusions:
- The water-in-deep eutectic solvent (DES) system provides a sustainable and efficient platform for producing alginate-derived carbon (AAC) materials.
- AAC materials exhibit excellent protein-friendly characteristics, enhancing both the activity and stability of immobilized enzymes like Cyt-c.
- The developed AAC materials hold promise for diverse biotechnological applications requiring stable and active enzyme immobilization.
