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A simple method for determination of plasma thiocyanate.
M Falkensson1, P Lundquist, H Rosling
1Department of Clinical Chemistry, Faculty of Health Sciences, Linköping University, Sweden.
Annals of Clinical Biochemistry
|July 1, 1988
Summary
A new colorimetric method determines thiocyanate in plasma from field-collected blood. This simple technique uses the König reaction but is unsuitable for urine due to interfering compounds.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Biochemistry
Background:
- Accurate thiocyanate determination is crucial for monitoring various physiological and toxicological conditions.
- Existing methods may be complex or unsuitable for rapid field analysis.
- Plasma thiocyanate levels provide insights into cyanide exposure and metabolic status.
Purpose of the Study:
- To develop and describe a straightforward method for quantifying thiocyanate in plasma.
- To adapt the method for blood samples collected under field conditions.
- To assess the applicability and limitations of the method.
Main Methods:
- Blood samples were collected in heparinised glass capillary tubes under field conditions.
- Plasma was separated and deproteinised using perchloric acid.
- Thiocyanate was quantified colorimetrically via the König reaction, employing sodium hypochlorite and barbituric acid.
Main Results:
- A reliable colorimetric method for plasma thiocyanate determination was successfully established.
- The method is suitable for analyzing samples collected in field settings.
- The method was found to be inapplicable to urine samples due to interfering substances.
Conclusions:
- The described König reaction-based colorimetric method offers a simple approach for plasma thiocyanate analysis.
- This method is advantageous for field-based sample collection and analysis.
- Further method development is required for accurate thiocyanate determination in urine.