Prevotella hominis sp. nov., isolated from human faeces

A strictly anaerobic predominant bacterium, designated as strain gm001^T, was isolated from a freshly voided faecal sample collected from a healthy Taiwanese adult. Cells were Gram-stain-negative rods, non-motile and non-spore-forming. Strain gm001^T was identified as a member of the genus Prevotella, and a comparison of 16S rRNA and hsp60 gene sequences revealed sequence similarities of 98.5 and 93.3 %, respectively, demonstrating that it was most closely related to the type strain of Prevotella copri. Phylogenomic tree analysis indicated that the gm001^T cluster is an independent lineage of P. copri DSM 18205^T. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between strain gm001^T and P. copri DSM 18205^T were 80.9, 28.6 and 83.8 %, respectively, which were clearly lower than the species delineation thresholds. The species-specific genes of this novel species were also identified on the basis of pan-genomic analysis. The predominant menaquinones were MK-11 and MK-12, and the predominant fatty acids were anteiso-C_15 : 0, C_15 : 0 and iso-C_15 : 0. Acetate and succinate were produced from glucose as metabolic end products. Taken together, the results indicate that strain gm001^T represents a novel species of the genus Prevotella, for which the name Prevotella hominis sp. nov. is proposed. The type strain is gm001^T (=BCRC 81118^T=JCM 33280^T).