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A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
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H2S and reactive sulfur signaling at the host-bacterial pathogen interface
Brenna J C Walsh1, David P Giedroc2
1Department of Chemistry, Indiana University, Bloomington, Indiana, USA.
The Journal of Biological Chemistry
|July 24, 2020
Summary
Hydrogen sulfide (H2S) and reactive sulfur species (RSS) protect bacteria against host defenses and antibiotics. This review explores how bacteria maintain H2S/RSS balance and how this impacts survival during infection.
Area of Science:
- Microbiology and Immunology
- Biochemistry and Molecular Biology
Background:
- Bacterial pathogens face host defenses like reactive oxygen and nitrogen species, plus antibiotic-induced stress.
- Hydrogen sulfide (H2S) and downstream reactive sulfur species (RSS) are increasingly recognized for their cytoprotective roles in bacteria.
Purpose of the Study:
- To review recent findings on the impact of H2S/RSS on bacterial survival in host environments.
- To elucidate mechanisms of RSS biogenesis, clearance, and homeostasis in bacteria.
- To discuss bacterial sensing of RSS and the role of proteome persulfidation in H2S/RSS signaling.
Main Methods:
- Literature review of studies investigating bacterial responses to host stressors.
- Discussion of proposed models for bacterial H2S/RSS homeostasis.
- Overview of fluorescence imaging and mass spectrometry techniques for RSS detection.
Main Results:
- H2S/RSS significantly influence bacterial survival in infected cells and animals.
- Bacterial transcriptional regulators act as sensors to maintain H2S/RSS homeostasis.
- Proteome persulfidation (S-sulfuration) is a potential mediator of bacterial H2S/RSS signaling.
Conclusions:
- Bacterial H2S/RSS homeostasis is crucial for adapting to host-imposed stresses.
- Understanding H2S/RSS signaling pathways, including persulfidation, is key to deciphering bacterial survival mechanisms.
- Further research is needed to fully understand the regulatory significance of proteome persulfidation in bacteria.
Keywords:
S-sulfurationantibiotic resistanceantibioticscysteinehost-pathogen interfacehydrogen sulfidemicrobial pathogenesismicrobiologypersulfidationpersulfidepost-translational modification (PTM)proteomicsreactive sulfur speciessulfhydrylthiol chemistryMore Related Videos
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