Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

1.9K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.9K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

14.3K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.3K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

8.2K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
8.2K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

15.5K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
15.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of the Cellular Immune Response to Group B Streptococcal Vaginal Colonization.

bioRxiv : the preprint server for biology·2025
Same author

A Distinctive γδ T Cell Repertoire in NOD Mice Weakens Immune Regulation and Favors Diabetic Disease.

Biomolecules·2022
Same author

Becoming aware of γδ T cells.

Advances in immunology·2022
Same author

γδ T Cells Activated in Different Inflammatory Environments Are Functionally Distinct.

Journal of immunology (Baltimore, Md. : 1950)·2022
Same author

BW5147 and Derivatives for the Study of T Cells and their Antigen Receptors.

Archivum immunologiae et therapiae experimentalis·2020
Same author

γδ T cells shape memory-phenotype αβ T cell populations in non-immunized mice.

PloS one·2019

Related Experiment Video

Updated: Dec 14, 2025

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

53.9K

Two functionally distinct subsets of IL-17 producing γδ T cells.

Rebecca L O'Brien1,2, Willi K Born1,2

  • 1Department of Biomedical Research, National Jewish Health, Denver, CO, USA.

Immunological Reviews
|July 24, 2020
PubMed
Summary

Gamma delta T-17 cells are crucial for immunity. In mice, two distinct types exist, with different developmental pathways for IL-17 production, unlike humans.

Keywords:
IL-17IL-22host defensethymic developmenttype 17 cytokinestype 3 immunityγδ T cell memoryγδ T cellsγδT-17 cells

More Related Videos

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

4.0K
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

35.2K

Related Experiment Videos

Last Updated: Dec 14, 2025

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

53.9K
Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

4.0K
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

35.2K

Area of Science:

  • Immunology
  • Cellular Biology
  • T-cell research

Background:

  • Gamma delta (γδ) T cells are vital immune cells, producing Interleukin-17 (IL-17), a key cytokine for pathogen resistance.
  • In mice, IL-17-producing γδ T cells (γδT-17) consist of two subsets defined by T-cell receptor (TCR) expression: Vγ6Vδ1+ and Vγ4+.
  • Murine γδT-17 cells develop a predisposition for IL-17 production during thymic maturation.

Purpose of the Study:

  • To review the similarities and differences between the two distinct types of murine γδT-17 cells.
  • To discuss the potential implications arising from the distinct characteristics of these γδT-17 cell subsets.
  • To explore the evidence for analogous IL-17-producing γδ T cell subsets in humans and their developmental differences.

Main Methods:

  • Review of existing literature on γδ T cell subsets and IL-17 production.
  • Comparative analysis of TCR Vγ and Vδ chain expression in murine γδT-17 cells.
  • Examination of evidence for distinct γδ T cell subsets in human immunity.

Main Results:

  • Two distinct subsets of γδT-17 cells exist in mice, characterized by invariant Vγ6Vδ1+ or Vγ4+ TCRs.
  • Murine γδT-17 cells are imprinted for IL-17 production during thymic development.
  • Evidence suggests two similar subsets in humans, but they likely acquire IL-17 bias in the periphery, not the thymus.

Conclusions:

  • The distinct developmental pathways of murine γδT-17 cell subsets may have significant implications for immune responses.
  • Understanding these differences is crucial for comprehending γδ T cell heterogeneity and function.
  • Human IL-17-producing γδ T cells may differ fundamentally in their developmental programming compared to mice.