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Updated: Dec 14, 2025

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in
Vladimir Pekarik1,2, Marie Peskova3, Jakub Duben4
1Institute of Physiology, Faculty of Medicine, Masaryk University, 625 00, Brno, Czech Republic. pekarikv@mail.muni.cz.
Abstract:
Allyl- and propargyl ethers of umbelliferone are sensitive probes for palladium and platinum, including anticancer compounds cisplatin, carboplatin and oxaliplatin, and effective for direct visualization of protein and DNA complexes with organometallic compounds in polyacrylamide gels allowing easy detection of interactions with analyzed protein or nucleic acid. Both probes can be used for fast evaluation of Pd/Pt binding to nanocarriers relevant in drug targeted therapy or specific clinically relevant target macromolecules.

