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Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

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Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
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T Cell Activation and Clonal Selection01:22

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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T Cell Types and Functions01:24

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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Related Experiment Video

Updated: Dec 13, 2025

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

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T Cell Receptor Repertoire Sequencing.

Huixin Lin1, Yonggang Peng1, Xiangbin Chen1

  • 1Geneis (Beijing) Co., Ltd., Beijing, People's Republic of China.

Methods in Molecular Biology (Clifton, N.J.)
|July 26, 2020
PubMed
Summary
This summary is machine-generated.

T cell receptor (TCR) profiling using high-throughput sequencing is crucial for understanding immune responses and disease progression. This method aids in disease monitoring and prognosis prediction, offering valuable insights into immune system health.

Keywords:
Data analysisHigh-throughput sequencingLibrary constructionT cell receptor repertoire

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Last Updated: Dec 13, 2025

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Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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Area of Science:

  • Immunology
  • Genomics
  • Biotechnology

Background:

  • T cell receptor (TCR) repertoire status is linked to various diseases.
  • TCR profiling is essential for monitoring immune responses and predicting disease prognosis.

Purpose of the Study:

  • To provide a comprehensive overview of T cell receptor (TCR) profiling.
  • To detail the methodologies involved in high-throughput sequencing for TCR repertoire analysis.

Main Methods:

  • DNA extraction from biological samples.
  • Library construction for high-throughput sequencing.
  • High-throughput sequencing and subsequent data analysis.

Main Results:

  • Established protocols for TCR profiling from sample to data analysis.
  • Demonstrated the utility of high-throughput sequencing in TCR repertoire studies.

Conclusions:

  • TCR profiling is a powerful tool in medical research and diagnostics.
  • High-throughput sequencing significantly advances the study of TCR repertoires and their clinical relevance.