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Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
Transfection of Small Noncoding RNAs into Arabidopsis thaliana Protoplasts
Stéphanie Lalande1, Marjorie Chery1, Elodie Ubrig1
1Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, Strasbourg, France.
Abstract:
Polyethylene glycol transfection of plant protoplasts represents an efficient method to incorporate foreign DNA and study transient gene expression. Here, we describe an optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts. An example of application is provided by demonstrating the incorporation of a 20 nt long small noncoding RNA deriving from the 5' extremity of an A. thaliana cytosolic alanine tRNA into freshly isolated protoplasts.
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