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Updated: Dec 13, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
Published on: September 8, 2023
The importance of cycle threshold values in interpreting molecular tests for SARS-CoV-2
Richard J Drew1, Sinéad O'Donnell2, David LeBlanc3
1Clinical Innovation Unit, Rotunda Hospital, Dublin, Ireland; Irish Meningitis and Sepsis Reference Laboratory, Children's Health Ireland at Temple Street, Dublin, Ireland; Royal College of Surgeons in Ireland, Dublin, Ireland.
Abstract:
Using the Allplex™ 2019-nCoV assay (Seegene, South Korea), 285 samples were tested; 49 (17%) were positive for 3 genes, 4 (1.4%) samples were positive for 2 genes (all N gene and RdRP gene), 8 (3%) samples were positive for 1 gene (all N gene only), and 224 (78.5%) samples were negative.
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