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Effect of Ultramicro Superparamagnetic Iron Oxide Nanoparticles on Cerebral Infarction in Mice
Bin Zhang1, Xiuting Di2, Yizhou Song1
1Department of Neurology, The Third Hospital of Liaocheng, Liaocheng 252000, Shandong, PR China.
Abstract:
To investigate the effect of Feraheme (ferumoxytol) intravenous injection on cerebral infarction volume and inflammatory response in mice with permanent middle cerebral artery occlusion. We randomly divided 30 CS7BL6J mice into sham operated group, normal saline control group, and Feraheme group with 10 mice in each group. The model of permanent occlusion of right middle cerebral artery was made via the modified suture method in the normal saline control group and the Feraheme group. After 24 h of establishment the model, the tail vein was injected with 18 mg/kg Feraheme in the sham operation group and Feraheme group, and the normal saline control group was injected with an equal volume of normal saline. Neurobehavioral scores were obtained 24 h (before injection of Feraheme or normal saline) and 48 h (before MRI) after the model was established. The volume of cerebral infarction was calculated according to T2 weighted imaging. Orbital blood was collected after nodal scanning to detect serum TNF-α, IL-1β, and IL-6 levels. Then, the brain tissues of mice were killed for HE staining and IBAL immunohistochemical staining. No significant differences in cerebral infarction volume and neurological function were observed between the normal saline control group and Feraheme group. The levels of TNF-α, IL-1β and IL-6 in the normal saline control group and Feraheme group were significantly higher than those in the sham operation group (P < 0.05), but there were no significant differences between the normal saline control group and Feraheme group. We showed that intravenous injection of 18 mg/kg Feraheme 24 h after cerebral ischemia does not affect the infarct volume and inflammatory response, suggesting that the dose of Feraheme can be used for molecular imaging studies of inflammatory response after cerebral ischemia.
Insights
Feraheme (ferumoxytol) intravenous injection did not alter cerebral infarction volume or inflammatory markers in mice following middle cerebral artery occlusion. This suggests Feraheme
Area of Science:
- Neuroscience
- Pharmacology
- Immunology
Background:
- Cerebral infarction, a major cause of disability, involves complex inflammatory processes.
- Iron oxide nanoparticles, like Feraheme (ferumoxytol), are used in medical imaging and may modulate inflammatory responses.
Purpose of the Study:
- To evaluate the impact of intravenous Feraheme on infarct volume and neuroinflammation in a mouse model of cerebral ischemia.
- To determine if Feraheme administration affects key inflammatory cytokines post-stroke.
Main Methods:
- A permanent middle cerebral artery occlusion model was established in C57BL6J mice.
- Mice received either Feraheme (18 mg/kg) or normal saline intravenously 24 hours post-occlusion.
- Cerebral infarct volume was assessed using T2-weighted MRI, and serum levels of TNF-α, IL-1β, and IL-6 were measured.
Main Results:
- No significant differences in cerebral infarct volume or neurological scores were observed between the Feraheme and saline groups.
- While both Feraheme and saline groups showed elevated inflammatory cytokines compared to sham controls, there were no significant differences between the Feraheme and saline groups.
- Histopathological analysis (HE and IBAL staining) also revealed no significant impact of Feraheme on brain tissue inflammation.
Conclusions:
- Intravenous Feraheme at 18 mg/kg, administered 24 hours after cerebral ischemia, does not significantly affect infarct volume or the acute inflammatory response in mice.
- These findings support the potential use of this Feraheme dosage for molecular imaging studies investigating post-cerebral ischemia inflammation.
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