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Updated: Dec 13, 2025

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
Demonstration of a Ribozyme in Epsilon Domain of Hepatitis B Virus RNA
Dibyajnan Chakraborty1, Sagarmoy Ghosh2
1Dr. KPC Life Sciences Pvt. Ltd., Kolkata, West Bengal, India.
Abstract:
The epsilon domain of Hepatitis B virus plays a crucial role in encapsidation of viral pregenomic RNA and its partial NMR structure has been determined. However, we recently described a potassium-dependent ribonucleolytic activity associated with this region, so that a 53 nt long RNA containing the epsilon domain could release itself and cleaved other RNAs. We describe here the experimental methodologies for setting up the reactions and outline a general strategy for initial demonstration of this self-cleaving ribozyme activity.
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