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Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo

Max Pietschmann1, Gregor Tempel1, Maral Halladjian1

  • 1Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|July 27, 2020
PubMed
Summary

Lariat capping, a novel mRNA cap substitute, is installed by a ribozyme in certain microorganisms. Protocols are presented to validate lariat caps and measure ribozyme cleavage efficiency in vivo.

Keywords:
IRESLariat capLariat capping ribozymeSpongingm7G-cap

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