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Related Concept Videos

Ribozymes02:47

Ribozymes

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The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
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Ribozymes02:47

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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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Updated: Dec 13, 2025

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
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Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

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Long Non-coding RNA Depletion Using Self-Cleaving Ribozymes.

Alex C Tuck1, Marc Bühler2,3

  • 1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|July 27, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel ribozyme method to deplete long non-coding RNA (lncRNA) products in mouse stem cells. This technique allows for precise testing of the functional roles of specific lncRNA molecules.

Keywords:
Genome editingLong non-coding RNARNARibozymelincRNAlncRNA

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DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
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Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Long non-coding RNAs (lncRNAs) have diverse biological functions, acting as functional RNA molecules, DNA elements, or through transcriptional regulation.
  • Understanding the specific roles of lncRNA products is crucial for deciphering their contribution to cellular processes.
  • Existing methods for studying lncRNA function can be limited in their specificity and impact on the host gene.

Purpose of the Study:

  • To develop and validate a novel ribozyme-based strategy for the targeted depletion of endogenous long non-coding RNA (lncRNA) products.
  • To enable functional studies of lncRNA products by specifically removing them with minimal perturbation of their encoding gene.
  • To provide a tool for investigating the roles of lncRNAs in mouse embryonic stem cells.

Main Methods:

  • Utilized a ribozyme-based approach for targeted degradation of endogenous lncRNA molecules.
  • Applied the method in mouse embryonic stem cells to assess its efficacy and specificity.
  • Ensured minimal disruption to the lncRNA gene locus during the depletion process.

Main Results:

  • Successfully depleted the target endogenous lncRNA product in mouse embryonic stem cells.
  • Demonstrated that the ribozyme-based depletion minimally impacted the lncRNA gene itself.
  • Established a viable method for functional analysis of lncRNA products.

Conclusions:

  • The developed ribozyme-based approach offers a powerful and specific tool for studying lncRNA function.
  • This method facilitates the investigation of lncRNA product roles by enabling targeted depletion.
  • The technique is applicable to mouse embryonic stem cells, paving the way for further research into lncRNA biology.