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Updated: Dec 13, 2025

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Retinal changes in mice spontaneously developing diabetes by Th17-cell deviation.

Manzo Taguchi1, Hideaki Someya1, Makoto Inada1

  • 1Department of Ophthalmology, National Defense Medical College (NDMC), Namiki 3-2, Tokorozawa, Saitama, 359-0042, Japan.

Experimental Eye Research
|July 28, 2020
PubMed
Summary
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Interleukin-17 (IL-17) plays a role in diabetic retinopathy (DR) development. This study found that T helper 17 (Th17) cell responses promote retinal changes in diabetic mice, suggesting a link between Th17 immunity and DR pathogenesis.

Area of Science:

  • Immunology
  • Ophthalmology
  • Endocrinology

Background:

  • Elevated interleukin-17 (IL-17) levels are implicated in diabetic retinopathy (DR).
  • The precise role of IL-17 in DR pathogenesis remains unclear.
  • T helper 17 (Th17) cells are a primary source of IL-17.

Purpose of the Study:

  • To investigate the involvement of Th17-mediated immune responses in the pathogenesis of diabetic retinopathy.
  • To analyze the impact of interferon-gamma deficiency on Th17 cell activation and DR development in diabetic mice.

Main Methods:

  • Established Ins2Akita IFN-γ-deficient (Akita-GKO) mice by crossbreeding Akita and GKO mice.
  • Assessed blood glucose levels (BGL), Th17 cell proportions, and gene expression (ROR-γt, GATA-3, VEGF, ICAM-1).
Keywords:
Diabetic retinopathyIL-17AT cell mediated immunityTh17 activationTh17 cell differentiation

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  • Evaluated retinal vascular changes including leukostasis, VEGF protein levels, edema, exudates, and basement membrane thickening.

    • Main Results:

    • Akita-GKO mice exhibited significantly higher proportions of IL-17 and IL-22-producing splenic CD4+ cells compared to other groups.
    • Retinal expression of VEGF and ICAM-1, along with ocular VEGF protein levels and leukostasis, were significantly increased in Akita-GKO mice.
    • Akita-GKO mice showed retinal edema, exudative lesions, and vascular basement membrane thickening, indicative of DR progression.

    Conclusions:

    • Th17 cell-mediated immune responses are implicated in promoting functional and morphological changes in the retina of diabetic mice.
    • These findings suggest that Th17 cells contribute to the pathogenesis of diabetic retinopathy.
    • Targeting Th17 pathways may offer a therapeutic strategy for managing diabetic retinopathy.