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Circular RNA DHX33 promotes malignant behavior in ccRCC by targeting miR-489-3p/MEK1 axis

Jie Wang1,2, Jian-Qiu Zhang1,2, Xiao-Lei Zhao3

  • 1Department of Urology, Ninghai First Hospital, Zhejiang 315600, China.

Aging
|July 28, 2020
PubMed

Insights

A novel circular RNA, circDHX33, promotes clear cell renal cell carcinoma (ccRCC) progression by regulating the miR-489-3p/MEK1 pathway. Targeting circDHX33 may offer a new therapeutic strategy for ccRCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer development.
  • The specific involvement of circRNAs in the progression of clear cell renal cell carcinoma (ccRCC) remains largely unexplored.

Purpose of the Study:

  • To investigate the function and mechanism of a novel circRNA, circDHX33, in ccRCC.
  • To determine if circDHX33 could serve as a potential therapeutic target for ccRCC.

Main Methods:

  • Quantitative real-time PCR to measure circDHX33 expression in ccRCC cell lines and tissues.
  • Cell proliferation and invasion assays.
  • In vivo tumor growth experiments.
  • Mechanistic studies involving microRNA (miRNA) sponging and gene expression analysis.

Main Results:

  • circDHX33 was found to be upregulated in ccRCC cell lines and patient tissues.
  • Higher circDHX33 levels correlated with advanced TNM stage and metastasis in ccRCC patients.
  • Inhibition of circDHX33 suppressed ccRCC cell proliferation, invasion, and tumor growth in vivo.
  • circDHX33 was shown to promote ccRCC progression by sponging miR-489-3p and consequently modulating MEK1 expression.

Conclusions:

  • circDHX33 acts as an oncogenic circRNA in ccRCC progression.
  • The circDHX33/miR-489-3p/MEK1 axis is a key pathway driving ccRCC malignancy.
  • circDHX33 represents a promising novel therapeutic target for ccRCC.
Keywords:
MEK1circDHX33clear cell renal cell carcinomamiR-489-3p

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