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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Published on: September 19, 2017

Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose.

Nicolae-Bogdan Mincu^1,2, Veronica Lazar¹, Dana Stan²

¹Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania.

Diagnostics (Basel, Switzerland)

|July 30, 2020

Summary

Rapid diagnostic methods are crucial for diseases like cancer and infections. Screen-printed electrode biosensors offer sensitive and selective detection of biomarkers and pathogens, aiding in timely diagnosis.

Keywords:

biosensors diagnosis screen-printed electrodes

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Area of Science:

Electrochemistry
Biosensor Technology
Biomarker Detection

Background:

Increasing prevalence of infectious diseases, cancer, and metabolic syndrome necessitates advanced diagnostic tools.
Screen-printed electrodes (SPEs) offer a promising platform for developing sensitive, selective, and cost-effective biosensors.
Various electrode materials (carbon, gold, platinum) present unique advantages and challenges for biosensor functionalization.

Purpose of the Study:

To review recent advancements in screen-printed electrode-based biosensors for disease diagnosis.
To highlight promising biosensor designs for detecting proteins, biomarkers, and pathogenic microorganisms.
To discuss the functionalization strategies and material choices for SPEs in biosensing applications.

Main Methods:

Comprehensive literature review of biosensors utilizing screen-printed electrodes.
Analysis of different electrode materials and their impact on biosensor performance.
Evaluation of biosensor applications in detecting specific disease markers and pathogens.

Main Results:

SPE-based biosensors demonstrate high sensitivity, selectivity, and low detection limits for various analytes.
Successful detection of key proteins, cancer biomarkers, and infectious agents has been reported.
Material selection and surface functionalization are critical for optimizing biosensor efficacy.

Conclusions:

Screen-printed electrode biosensors represent a powerful tool for rapid and accurate disease diagnostics.
Continued research into novel materials and functionalization techniques will further enhance biosensor capabilities.
SPE biosensors hold significant potential for point-of-care testing and widespread clinical application.