Related Experiment Video

Updated: Dec 13, 2025

Assessing Whole-Body Lipid-Handling Capacity in Mice
07:57

Assessing Whole-Body Lipid-Handling Capacity in Mice

Published on: November 24, 2020

4.5K

Predictors of oxylipins in a healthy pediatric population

Teresa Buckner1, Lauren A Vanderlinden1, Randi K Johnson1

  • 1University of Colorado Denver Anschutz Medical Campus, Denver, CO, USA.

Pediatric Research
|July 30, 2020
PubMed

Insights

In children at risk of type 1 diabetes, oxylipin levels change with age and are linked to precursor fatty acids in plasma. Omega-3 oxylipins show stronger associations with diet and red blood cell levels than omega-6 oxylipins.

Area of Science:

  • Biochemistry
  • Pediatric Endocrinology
  • Inflammation Research

Background:

  • Oxylipins, derived from omega-6 (n6) and omega-3 (n3) fatty acids, play roles in inflammation, primarily studied in adults.
  • Understanding oxylipin dynamics in children is crucial, especially those at risk for type 1 diabetes.

Purpose of the Study:

  • To investigate predictors of oxylipins in healthy children at risk of type 1 diabetes.
  • To examine associations between oxylipins, precursor fatty acids, diet, and environmental factors in children.

Main Methods:

  • Measured 27 n6- and 12 n3-oxylipins using LC-MS/MS in plasma from 111 children (age 1-17).
  • Assessed precursor fatty acids in plasma and red blood cell (RBC) membranes.
  • Collected dietary intake and environmental tobacco smoke (ETS) data via questionnaires.
  • Employed linear mixed models to analyze relationships.

Main Results:

  • Age was associated with numerous n6 and n3 oxylipins.
  • Oxylipins correlated more frequently with plasma precursor fatty acids than RBC levels or dietary intake.
  • Omega-3 oxylipins showed stronger associations with RBC levels and dietary precursor fatty acids compared to omega-6 oxylipins.
  • Environmental tobacco smoke exposure was linked to lipoxin A4 levels.

Conclusions:

  • Oxylipin levels in healthy children are influenced by age.
  • Plasma precursor fatty acid levels appear to be a key determinant of oxylipin generation in children.
  • The relationship between oxylipins and their precursors differs between omega-3 and omega-6 fatty acids, with stronger links observed for n3-oxylipins to diet and RBC levels.
Abstract

More Related Videos

Shotgun Lipidomics of Rodent Tissues11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.5K
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.6K

Related Concept Videos

Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
6.0K
Pharmacokinetics in Pediatric Patients: Drug Distribution01:17

Pharmacokinetics in Pediatric Patients: Drug Distribution

Drug distribution in the pediatric population exhibits unique challenges and considerations due to the physiological differences between children, particularly neonates and infants, and adults. A crucial aspect of pediatric pharmacology is understanding how these differences impact the pharmacokinetics of various drugs, necessitating age-specific dosing strategies to ensure efficacy and safety.Neonates and infants have a higher total body water content, ~75%–90% of their body weight,...
176
Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
19.4K
Pharmacokinetics in Pediatric Patients: Drug Metabolism01:24

Pharmacokinetics in Pediatric Patients: Drug Metabolism

In pediatric care, understanding the nuances of hepatic drug metabolism is crucial, as it significantly differs from that of adults. This divergence is primarily due to the developmental stage of drug-metabolizing enzymes, which affects how medications are processed in the body. In neonates, for instance, the activity of Phase I enzymes—critical for the initial breakdown of drugs—is markedly reduced, functioning at just 20–40% of the levels seen in adults. This reduction poses...
113
Pharmacokinetics in Pediatric Patients: Overview and Drug Absorption01:23

Pharmacokinetics in Pediatric Patients: Overview and Drug Absorption

Understanding the physiological differences in the pediatric population is crucial for effective pharmacotherapy. Neonates, infants, and children exhibit significant variations in gastric pH, gastric emptying time, intestinal transit time, and biliary function. These variations profoundly affect oral drug absorption, necessitating a nuanced approach to pediatric dosing.Neonates present with a unique physiological profile, having a gastric pH greater than 4 and faster and more irregular gastric...
148
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
1.1K