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Updated: Dec 13, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
Published on: April 21, 2022
Substitutional RNA Editing in Plant Organelles
Mizuho Ichinose1,2, Mamoru Sugita3
1Center for Gene Research, Nagoya University, Nagoya, Japan. miz-ichi@agr.kyushu-u.ac.jp.
Abstract:
RNA editing by cytidine (C) to uridine (U) conversions frequently occurs in land plant mitochondria and plastids. Target cytidines are specifically recognized by nuclear-encoded pentatricopeptide repeat (PPR) proteins in a sequence-specific manner. In the moss Physcomitrella patens, all PPR editing factors possess the DYW-deaminase domain at the C-terminus. Here, we describe methods for the direct sequencing of cDNA to detect RNA editing events and the RNA electrophoresis mobility shift assay (REMSA) to analyze the specific binding of PPR editing factors to their target RNA.
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