Cytocompatibility of filling pastes by primary teeth root simulating model

Evaluate the cytocompatibility of Calen^®/ZO, Calcicur^®, Vitapex^®, Endoflas^®, and zinc oxide/eugenol-based (ZOE) root canal pastes (RCP) to human primary osteoblasts (HPO) through a simplified model for primary teeth. The model employed pipette tips filled with 0.037 g of paste, exposed to 185 µL of culture medium for 24 h (n = 6). Release of components was analysed by Proton Nuclear Magnetic Resonance Spectroscopy (¹H-NMR). HPO were exposed to conditioned media for 24 h. Cell viability was assessed by cell density and metabolic activity, and release of interleukin 6 (IL-6), vascular endothelial growth factor (VEGF) and fibroblast growth factor (bFGF) by immunological assay. Physicochemical properties and antimicrobial efficacy were also evaluated. ¹H-NMR spectra analysis showed similarity between ZOE, Endoflas^®, Calcicur^®, and Vitapex^® compared to Calen^®/ZO and positive control, which showed distinct released components. Calen^®/ZO and Calcicur^® exhibited high alkaline pH in all periods and showed similar solubility. Calen^®/ZO, ZOE, and Vitapex^® showed similar flow rate. Calen^®/ZO, Calcicur^®, and Vitapex^® did not exhibit antimicrobial efficacy. Calen^®/ZO presented cytotoxicity (p < 0.05). Pastes did not increase IL-6 release compared to control. Apart from Vitapex^®, all pastes significantly induced VEGF/bFGF release. Interactive effects among released products may affect biological response to filling pastes. Calcicur^®, ZOE, Endoflas^® and Calen^®/ZO presented good to moderate cytocompatibility, with low impact on pro-inflammatory cytokine release and induction of growth factors of interest to tissue repair. This simplified model, specific for the evaluation of the cytocompatibility of RCPs on primary teeth, suggests how these pastes might contribute to bone repair in clinical situations of apical periodontitis in children.