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Cytocompatibility of filling pastes by primary teeth root simulating model
Andréa Vaz Braga Pintor1, Luciana Domênico Queiroz2, Mariana Coutinho Sancas1
1Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rua Prof Rodolpho Paulo Rocco, 325, Ilha do Fundão, Rio de Janeiro, 21941-971, Brazil.
Odontology
|August 1, 2020
Summary
This study evaluated root canal pastes for primary teeth. Most pastes showed moderate cytocompatibility and low inflammatory response, suggesting potential for bone repair in children with apical periodontitis.
Area of Science:
- Biomaterials Science
- Dental Research
- Cell Biology
Background:
- Root canal pastes (RCPs) are crucial in pediatric endodontics.
- Evaluating their cytocompatibility with primary human osteoblasts (HPO) is vital for bone repair.
- A simplified model for primary teeth is needed to assess RCP performance.
Purpose of the Study:
- To assess the cytocompatibility of five RCPs (Calen®/ZO, Calcicur®, Vitapex®, Endoflas®, and zinc oxide/eugenol-based) with HPO.
- To analyze component release and its effect on HPO viability and cytokine profiles.
- To evaluate physicochemical properties and antimicrobial efficacy of the RCPs.
Main Methods:
- A simplified in vitro model using pipette tips filled with RCPs and exposed to culture medium.
- Proton Nuclear Magnetic Resonance Spectroscopy (¹H-NMR) for component release analysis.
- Assessing HPO viability, IL-6, VEGF, and bFGF release after exposure to conditioned media.
Main Results:
- ¹H-NMR revealed distinct component release profiles among pastes.
- Calen®/ZO and Calcicur® showed high alkaline pH and solubility; Calen®/ZO, ZOE, and Vitapex® had similar flow rates.
- Calen®/ZO, Calcicur®, and Vitapex® lacked antimicrobial efficacy; Calen®/ZO exhibited cytotoxicity.
- Most pastes induced VEGF/bFGF release without increasing IL-6, indicating potential for tissue repair.
Conclusions:
- Calcicur®, ZOE, Endoflas®, and Calen®/ZO demonstrated good to moderate cytocompatibility.
- These RCPs had minimal impact on pro-inflammatory cytokines and promoted growth factors relevant to tissue repair.
- The simplified model suggests these pastes may aid bone repair in pediatric apical periodontitis.
