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Related Experiment Video

Updated: Dec 13, 2025

Development of an In Vitro Ocular Platform to Test Contact Lenses

Development of an In Vitro Ocular Platform to Test Contact Lenses

Published on: April 6, 2016

A Biomimetic, Stem Cell-Derived In Vitro Ocular Outflow Model.

Yangzi Isabel Tian¹, Xulang Zhang¹, Karen Torrejon²

¹College of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY, 12203, USA.

Advanced Biosystems

|August 1, 2020

Summary

Human induced pluripotent stem cell-derived trabecular meshwork (TM) cells mimic primary cells on scaffolds. This offers a scalable source for glaucoma drug screening and understanding aqueous humor outflow.

Keywords:

3D culture glaucoma microfabrication outflow stem cell trabecular meshwork

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Last Updated: Dec 13, 2025

Development of an In Vitro Ocular Platform to Test Contact Lenses

Development of an In Vitro Ocular Platform to Test Contact Lenses

Published on: April 6, 2016

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Published on: August 6, 2020

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

Area of Science:

Ophthalmology
Stem Cell Biology
Biomaterials Science

Background:

Age-related and disease-related human trabecular meshwork (HTM) cell loss can impair aqueous humor outflow and elevate intraocular pressure, contributing to glaucoma.
Induced pluripotent stem cells (iPSCs) offer a potential source for autologous trabecular meshwork (TM) cells to restore TM cell population and function.

Purpose of the Study:

To evaluate the suitability of human iPSC-derived TM cells cultured on SU-8 scaffolds as a model for HTM cell biology and glaucoma research.
To assess the potential of this in vitro system for drug screening and understanding outflow pathway physiology.

Main Methods:

Human iPSC-derived TM cells were cultured on SU-8 scaffolds.
Characterization of cell morphology, extracellular matrix deposition, and drug responsiveness (to dexamethasone) was performed.

Main Results:

Human iPSC-derived TM cells cultured on SU-8 scaffolds demonstrated HTM-like cell morphology.
These cells exhibited extracellular matrix deposition characteristic of native HTM cells.
The cells showed appropriate drug responsiveness to dexamethasone treatment, similar to primary HTM cells.

Conclusions:

Human iPSC-derived TM cells cultured on SU-8 scaffolds represent a viable and reproducible in vitro model system.
This system provides a scalable cell source for glaucoma drug screening and advancing the understanding of outflow pathway physiology.
The findings support the potential for personalized medicine approaches in glaucoma treatment.