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Non-canonical lipoproteins with programmable assembly and architecture
Md Shahadat Hossain1, Corina Maller, Yinghui Dai
1Department of Chemistry, 1-014 Center for Science and Technology, Syracuse University, Syracuse, NY 13244, USA. dmozhdeh@syr.edu.
Chemical Communications (Cambridge, England)
|August 1, 2020
Summary
Researchers synthesized artificial lipoproteins with unique non-canonical post-translational modifications (ncPTMs). These novel ncPTMs enable programmable material assembly and offer new possibilities for nanomaterial design.
Area of Science:
- Biochemistry
- Materials Science
- Nanotechnology
Background:
- Acyltransferases typically modify proteins with canonical post-translational modifications (PTMs).
- Artificial lipoproteins are engineered biomolecules with potential applications in drug delivery and materials science.
- Understanding lipoprotein assembly is crucial for designing functional nanomaterials.
Purpose of the Study:
- To leverage acyltransferase substrate promiscuity for synthesizing artificial lipoproteins with non-canonical PTMs (ncPTMs).
- To investigate the assembly behavior of these novel lipoproteins and their potential for creating advanced materials.
- To explore the expansion of PTMs for novel nanomaterial development.
Main Methods:
- Utilizing the substrate promiscuity of an acyltransferase enzyme.
- Synthesizing artificial lipoproteins incorporating ncPTMs.
- Characterizing the assembly properties of the engineered lipoproteins.
- Fabricating hybrid multiblock materials with programmable amphiphilicity.
Main Results:
- Successfully synthesized artificial lipoproteins functionalized with ncPTMs.
- Observed distinctive hysteretic assembly behavior in ncPTM-bearing lipoproteins, unlike canonical ones.
- Demonstrated the preparation of hybrid multiblock materials with precise amphiphilicity patterns.
- Established a novel method for creating nanomaterials with unique assembly and function.
Conclusions:
- The study highlights the potential of ncPTMs in engineering lipoprotein assembly.
- This approach enables the creation of advanced hybrid materials with tailored properties.
- Expanding the PTM repertoire offers new avenues for designing functional nanomaterials.
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