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Updated: Dec 13, 2025
A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
A Scoping Review of Research on the Human Milk Microbiome
Maureen Wimberly Groer1,2,3,4, Katherine Hope Morgan4, Adetola Louis-Jacques1
17831 University of South Florida College of Nursing, Tampa, FL, USA.
Background:
The human milk microbiome is an emerging scientific area. Careful, accurate collection and measurement for microbial sequencing is imperative. There is controversy about a core microbiome, and little is known about factors that influence composition. Even less known are ways that the milk microbiome might seed the infant gut and affect health.
Research Aim:
The aim of this paper is to provide a critical appraisal of milk microbiome research. The four areas of critical appraisal were collection and measurement, composition, effects on composition, and potential health effects for infants related to the milk microbiome.
Methods:
Using a PRISMA-ScR scoping review, we reviewed sources of evidence extracted from PubMed, Web of Science, CINAHL, Academic Search Complete, and PSYCHINFO data sets using the following criteria: English language, published in past 6 years, primary data, and sequencing using Next Generation Sequencing. Charting of sources of evidence included authors, title, journal year, sample, design, and results. The research questions posed were: How is human milk collected and how are the microbes identified? What is the composition and what factors affect the human milk microbiome? What is the relationship of the human milk microbiome to infant biology and health?
Results:
The reviewed studies were quantitative, cross sectional, or longitudinal. A core microbiome may be present. The microbiome may seed the early infant gut and promote physiological functions and thus influence human health.
Conclusions:
We have suggested concerns about collection and measurement that lead to gaps in knowledge generation, and mechanistic studies are lacking.
