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Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
Published on: July 30, 2016
Antioxidative effect of melatonin on cryopreserved ovarian tissue in mice
Xiao Chao Liu1, Tie Cheng Sun2, Hui Ying Li3
1Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China; Department of Human Anatomy, Basic Medical College, Chengde Medical University, Hebei, 067000, China.
Abstract:
Cryopreservation of ovarian tissues (OTs) has become the most effective way to preserve the fertility of female cancer patients. However, cryopreservation of OTs is still relatively at an experimental stage. The aim of study is to examine the effect of melatonin (MTL) on cryopreserved-thawed OTs. Fragments of OTs were cryopreserved in medium containing different concentrations (0 mM, 0.001 mM, 0.01 mM, 0.1 mM and 1 mM) of MLT. The endogenous enzymes (GSH-PX, GSH, SOD, CAT and T-AOC), MDA and ROS levels were all evaluated after cryopreservation. Our results showed that the 0.1 mM of MLT significantly improved the survival and diameter of follicles (P < 0.001). Meanwhile, the antioxidant enzymes activities (including GSH-PX, GSH, SOD, CAT and T-AOC) were enhanced and MDA content were significantly decreased in 0.1 mM of MLT group compared to other groups (P < 0.001). Additionally, compared to the control group, MTL of 0.1 mM resulted in a significantly lower ROS level. In conclusion, MLT protects the quality of cryopreserved OTs by decreasing oxidative stress level and the optimal concentration is 0.1 mM.
Insights
Melatonin (MTL) significantly improves ovarian tissue (OT) survival after cryopreservation. The optimal MTL concentration of 0.1 mM reduces oxidative stress, preserving ovarian tissue quality for fertility preservation.
Area of Science:
- Reproductive Biology
- Cryobiology
- Biochemistry
Background:
- Ovarian tissue (OT) cryopreservation is crucial for female cancer patient fertility preservation.
- Current OT cryopreservation techniques remain experimental.
- Oxidative stress negatively impacts cryopreserved ovarian tissue viability.
Purpose of the Study:
- To investigate the protective effects of melatonin (MTL) on cryopreserved-thawed ovarian tissues.
- To determine the optimal concentration of MTL for improving ovarian tissue quality post-cryopreservation.
Main Methods:
- Ovarian tissue fragments were cryopreserved with varying concentrations of melatonin (0–1 mM).
- Evaluated key oxidative stress markers: malondialdehyde (MDA) and reactive oxygen species (ROS).
- Assessed endogenous antioxidant enzyme activities: glutathione peroxidase (GSH-PX), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC).
Main Results:
- 0.1 mM melatonin significantly enhanced follicle survival and diameter (P < 0.001).
- Melatonin treatment increased antioxidant enzyme activities and decreased MDA levels (P < 0.001).
- A significant reduction in ROS levels was observed with 0.1 mM melatonin compared to controls (P < 0.001).
Conclusions:
- Melatonin protects cryopreserved ovarian tissues by mitigating oxidative stress.
- The optimal concentration of melatonin for preserving ovarian tissue quality is 0.1 mM.
- Melatonin shows promise as a supplement for improving fertility preservation outcomes in cancer patients.
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