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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
Published on: October 7, 2025
231
Experimental and theoretical study for miR-155 detection through resveratrol interaction with nucleic acids using
Samira Yazdanparast1, Ali Benvidi2, Mostafa Azimzadeh3,4,5
1Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
Mikrochimica Acta
|August 3, 2020
Summary
A new electrochemical nanobiosensor detects miR-155, a breast cancer biomarker, using Fe3O4NPs@Ag nanoparticles and resveratrol as a label. This sensitive sensor shows high accuracy in real serum samples.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Electrochemistry
Background:
- MicroRNA-155 (miR-155) is a significant biomarker for breast cancer.
- Early and accurate detection of cancer biomarkers is crucial for effective treatment.
- Existing detection methods may lack sensitivity, specificity, or require complex procedures.
Purpose of the Study:
- To develop a novel, sensitive, and specific electrochemical nanobiosensor for detecting miR-155.
- To utilize Fe3O4NPs@Ag core-shell nanoparticles for enhanced sensor performance.
- To validate the sensor's efficacy in real biological samples.
Main Methods:
- Synthesis and characterization of Fe3O4NPs@Ag core-shell nanoparticles using SEM, FTIR, and XRD.
- Fabrication of a magnetic bar carbon paste electrode modified with anti-miR-155 probes.
- Detection of miR-155 hybridization using resveratrol as an electrochemical label and DPV analysis.
- Optimization and validation using CV, EIS, and testing with human serum samples.
Main Results:
- The nanobiosensor demonstrated a wide linear range (0.5 fM to 1.0 nM) with a low detection limit (0.15 fM).
- The sensor exhibited excellent reproducibility and specificity against mismatched sequences.
- Detection of miR-155 in spiked human serum samples showed no significant interference.
- Molecular dynamics simulations confirmed resveratrol's preferential binding to double-stranded DNA.
Conclusions:
- The developed electrochemical nanobiosensor offers a highly sensitive and specific platform for miR-155 detection.
- Fe3O4NPs@Ag nanoparticles and resveratrol labeling provide an effective strategy for miRNA-based biosensing.
- The sensor's performance in real samples highlights its potential for clinical applications in breast cancer diagnostics.
