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The Antisense long noncoding RNA AGAP2-AS1 regulates cell proliferation and metastasis in Epithelial Ovarian Cancer

Zheng Tingting1,2,3,4, Lin Xiaojing1,2,3, Tang Xiaoyan1,2,3

  • 1Department of Gynaecology, Obstetrics and Gynaecology Hospital, Fudan University, 419 Fangxie Road, Shanghai 200011, China Shanghai 200011, China.

Journal of Cancer
|August 4, 2020
PubMed

Insights

Antisense long noncoding RNA AGAP2-AS1 acts as a tumor suppressor in epithelial ovarian cancer (EOC). Its decreased expression correlates with advanced EOC, and it inhibits cancer progression by downregulating key genes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Antisense long noncoding RNAs (lncRNAs) regulate gene expression and are implicated in cancer.
  • AGAP2-AS1, an antisense lncRNA, is involved in various cancers, but its role in epithelial ovarian cancer (EOC) is unclear.

Purpose of the Study:

  • To investigate the clinical significance, biological functions, and regulatory mechanisms of AGAP2-AS1 in EOC.
  • To evaluate AGAP2-AS1 as a potential therapeutic target for EOC.

Main Methods:

  • Analysis of AGAP2-AS1 expression in EOC tissues and correlation with clinical parameters.
  • In vitro assays to assess the effects of AGAP2-AS1 on cell migration, invasion, and proliferation.
  • In vivo studies to evaluate tumor growth suppression by AGAP2-AS1.
  • Mechanistic studies to identify genes and pathways regulated by AGAP2-AS1.

Main Results:

  • AGAP2-AS1 expression was significantly decreased in EOC tissues compared to controls.
  • Low AGAP2-AS1 expression was associated with advanced FIGO stage, high histological grade, serous subtype, and lymph node metastasis.
  • AGAP2-AS1 inhibited EOC cell migration, invasion, and proliferation in vitro and suppressed tumor growth in vivo.
  • AGAP2-AS1 exerted its tumor-suppressive effects by downregulating KRAS, FGFR4, and CTSK, and suppressing epithelial-mesenchymal transition.

Conclusions:

  • AGAP2-AS1 functions as a tumor suppressor in EOC.
  • AGAP2-AS1 may serve as a valuable prognostic biomarker and a potential therapeutic target for EOC.
Keywords:
AGAP2-AS1epithelial ovarian cancermetastasisproliferation

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