QCM-D characterization of time-dependence of bacterial adhesion

Quartz crystal microbalance with dissipation monitoring (QCM-D) is becoming an increasingly popular technique that can be employed as part of experimental and modeling investigations of bacterial adhesion. The usefulness of QCM-D derives from this technique's ability to probe binding and interactions under dynamic conditions, in real time. Bacterial adhesion is an important first step in the formation of biofilms, the control of which is relevant to industries that include shipping, water purification, packaging, and biomedical devices. However, many questions remain unanswered in the bacterial adhesion process, despite extensive research in this area. With QCM-D, multiple variables affecting bacterial adhesion can be studied, including the roles of substrate composition, chemical modification, solution ionic strength, environmental temperature, shear conditions, and time. Recent studies demonstrate the utility of QCM-D in developing new bacterial adhesion models and studying different stages of biofilm formation. We provide a review of how QCM-D has been used to study bacterial adhesion at stages ranging from the first step of bacterial adhesion to mature biofilms, and how QCM-D studies are being used to promote the development of solutions to biofilm formation.