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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.0K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.0K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Related Experiment Video

Updated: Dec 13, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

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Combining high throughput and high quality for cryo-electron microscopy data collection.

Felix Weis1, Wim J H Hagen1

  • 1The Cryo-Electron Microscopy Service Platform, Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Acta Crystallographica. Section D, Structural Biology
|August 4, 2020
PubMed
Summary
This summary is machine-generated.

High-resolution cryo-electron microscopy (cryo-EM) now enables detailed 3D structure determination of macromolecular complexes. This review details advanced data collection methods for optimizing cryo-EM imaging and analysis.

Keywords:
coma-free imagingcryo-electron microscopyfringe-free imaginghigh-end data collection

More Related Videos

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

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Related Experiment Videos

Last Updated: Dec 13, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

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Area of Science:

  • Structural Biology
  • Biophysics
  • Microscopy

Background:

  • Cryo-electron microscopy (cryo-EM) is advancing rapidly, enabling high-resolution 3D structure determination of macromolecular complexes.
  • Technological progress in electron microscopes, detectors, and image processing has significantly improved cryo-EM capabilities.
  • New challenges in data collection, including microscope alignment and aberration correction, have emerged with higher resolution demands.

Purpose of the Study:

  • To review state-of-the-art data collection strategies for cryo-electron microscopy.
  • To present recent advancements in cryo-EM instrumentation and their impact on data acquisition.
  • To describe the optimization of imaging parameters for high-resolution cryo-EM data collection.

Main Methods:

  • Utilizing advanced electron microscopes with aberration correction.
  • Implementing high-throughput data acquisition workflows.
  • Optimizing imaging parameters such as magnification, electron dose, and defocus.

Main Results:

  • Recent microscope implementations enhance throughput while ensuring aberration-free imaging.
  • Optimized acquisition parameters facilitate the collection of high-resolution cryo-EM data.
  • The EMBL Heidelberg cryo-EM platform showcases advanced high-end data collection practices.

Conclusions:

  • High-end data collection is crucial for achieving high resolution in cryo-EM.
  • Continuous technological innovation and parameter optimization are key to advancing structural biology.
  • Efficient cryo-EM data collection protocols are essential for elucidating complex biological structures.