Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

199
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
199
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.8K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.8K
The Unfolded Protein Response01:37

The Unfolded Protein Response

6.0K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
6.0K
Improving Translational Accuracy02:07

Improving Translational Accuracy

13.6K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
13.6K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

16.2K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
16.2K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.3K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PRMT5 inhibition disrupts detained intron splicing and impairs ATR signaling with increased DNA damage.

Communications biology·2026
Same author

Activity-dependent lipid droplet biogenesis and turnover regulate synaptic integrity.

bioRxiv : the preprint server for biology·2026
Same author

Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion.

Science (New York, N.Y.)·2026
Same author

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same author

Targeted degradation of MDM2 overcomes feedback regulation of p53 signaling in Merkel cell carcinoma models.

The Journal of clinical investigation·2026
Same author

A human lysosomal storage disorder toolkit for decoding proteome landscapes in cortical-like and dopaminergic-like induced neurons.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Dec 13, 2025

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
08:07

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Published on: July 6, 2021

3.0K

EDF1 coordinates cellular responses to ribosome collisions.

Niladri K Sinha1, Alban Ordureau2, Katharina Best3

  • 1Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, United States.

Elife
|August 4, 2020
PubMed
Summary

Endothelial differentiation-related factor 1 (EDF1) is recruited to collided ribosomes, preventing the translation of defective mRNAs and regulating transcription. This discovery reveals new mechanisms in ribosome-mediated quality control.

Keywords:
biochemistrycell biologychemical biologyhumanquality controlribosomal collisionsribosometranscriptional response

More Related Videos

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.3K
Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

4.5K

Related Experiment Videos

Last Updated: Dec 13, 2025

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
08:07

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Published on: July 6, 2021

3.0K
Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.3K
Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

4.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Aberrant mRNA translation causes ribosomal collisions, activating cellular quality control pathways.
  • These pathways involve mRNA and nascent peptide degradation and ribosomal rescue mechanisms.

Purpose of the Study:

  • To systematically identify proteins associated with collided ribosomes.
  • To elucidate the role of Endothelial differentiation-related factor 1 (EDF1) in ribosome-mediated quality control.

Main Methods:

  • Sucrose gradient fractionation combined with quantitative proteomics to identify proteins on collided ribosomes.
  • Cryo-electron microscopy to determine the structural basis of EDF1-ribosome interaction.

Main Results:

  • Endothelial differentiation-related factor 1 (EDF1) was identified as a novel protein recruited to collided ribosomes.
  • EDF1 binds to the 40S ribosomal subunit near the mRNA entry channel and recruits translational repressors (GIGYF2, EIF4E2).
  • EDF1 initiates a negative-feedback loop to halt translation of defective mRNAs and regulates transcriptional responses.

Conclusions:

  • EDF1 plays a crucial role in coordinating multiple responses within the ribosome-mediated quality control pathway.
  • This study provides novel insights into the interplay between ribosome quality control and global transcriptional regulation.