Tungsten disulfide nanosheets-based colorimetric assay for glucose sensing
Yunfei Jiao1, Justin Li1, Junyi Xiang1
1Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Summary
This study presents a new colorimetric assay for glucose detection using glucose oxidase (GOx) and tungsten disulfide (WS2) nanosheets. The method offers sensitive and reliable glucose quantification in human serum samples.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- Glucose detection is crucial for diagnosing and managing diabetes.
- Existing methods may require complex procedures or expensive equipment.
- Development of sensitive and cost-effective glucose detection strategies is ongoing.
Purpose of the Study:
- To develop a novel colorimetric assay for glucose detection.
- To utilize the peroxidase-like activity of tungsten disulfide (WS2) nanosheets.
- To establish a sensitive and reliable method for glucose quantification.
Main Methods:
- A glucose oxidase (GOx)-mediated strategy was employed.
- Tungsten disulfide (WS2) nanosheets catalyzed the TMB-H2O2 reaction.
- Colorimetric signals were measured, correlating with hydrogen peroxide (H2O2) concentration.
Main Results:
- Glucose concentrations were directly proportional to absorbance.
- The assay detected glucose in the range of 1 nM–500 μM.
- A low limit of detection (0.1445 nM) was achieved.
- Satisfactory results were obtained for glucose detection in human serum.
Conclusions:
- The developed WS2-based colorimetric assay is effective for glucose detection.
- This method offers high sensitivity and reliability.
- The assay shows potential for practical applications, including clinical diagnostics.
Keywords:
3,3′,5,5′-TetramethylbenzidineColorimetric assayGlucose detectionPeroxidase mimeticWS(2) nanosheets
