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Updated: Dec 13, 2025

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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
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Current Progress on Antibiotic Sensing Based on Ratiometric Fluorescent Sensors.
Jinghan Liu1,2,3, Ying Li1,2,3, Lin Liu1,2,3
1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.
Bulletin of Environmental Contamination and Toxicology
|August 5, 2020
Summary
Overuse of veterinary antibiotics causes food contamination and human harm. Ratiometric fluorescent (RF) sensors offer a promising quantitative method for detecting these critical contaminants.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Food Safety
Background:
- Veterinary antibiotics are crucial for animal health but their overuse leads to significant food contamination and human health risks.
- Addressing antibiotic overuse necessitates advanced detection techniques for accurate quantification in food products.
Purpose of the Study:
- To review the construction strategies of recent ratiometric fluorescent (RF) sensors for antibiotic detection.
- To highlight recent advances and future trends in RF-based antibiotic sensing.
Main Methods:
- Focus on the development and application of ratiometric fluorescent (RF) sensing strategies.
- Analysis of various RF sensor designs for quantitative detection of antibiotics.
Main Results:
- RF techniques enable quantitative measurement by utilizing two distinct fluorescent emission intensities.
- Recent advancements show increasing potential for sensitive and selective antibiotic detection.
Conclusions:
- RF sensing presents a powerful approach for the quantitative analysis of antibiotics in food safety.
- Further research is needed to overcome challenges and expand opportunities in quantitative fluorescence sensing for antibiotics.

