Procyanidin B2 Improves Oocyte Maturation and Subsequent Development in Type 1 Diabetic Mice by Promoting...

Area of Science:

• Reproductive Biology
• Endocrinology
• Biochemistry

Background:

• Type 1 diabetes (T1D) impairs oocyte quality and early embryonic development.
• Procyanidin B2 (PB2), a grape seed extract, possesses potent antioxidant properties.

Purpose of the Study:

• To investigate the effects of PB2 on oocyte maturation in a mouse model of diabetes.
• To assess PB2's impact on oocyte quality, mitochondrial function, and developmental competence.

Main Methods:

• Diabetic mice were induced using streptozotocin (STZ).
• PB2 was added to the in vitro maturation medium for oocytes.
• Evaluated were germinal vesicle breakdown, polar body extrusion, reactive oxygen species (ROS), mitochondrial membrane potential, ATP levels, and histone crotonylation (crH4K5).

Main Results:

• PB2 significantly increased polar body extrusion (88.34% vs. 75.02%) and blastocyst rates (61.51% vs. 36.07%).
• PB2 reduced ROS generation (1.12 vs. 1.96) and improved mitochondrial function, including membrane potential (0.87 vs. 0.79 Δφm), ATP levels (1.31 vs. 0.71 pmol), and mitochondrial temperature.
• PB2 enhanced oocyte crotonylation at H4K5 (crH4K5) (47.26 vs. 59.68 pixels).

Conclusions:

• PB2 promotes oocyte maturation and viability in diabetic mice by enhancing mitochondrial function.
• PB2's ability to improve crH4K5 levels presents a novel therapeutic strategy for diabetes-induced oocyte quality decline.