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Updated: Dec 13, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
CRISPR/Cas: A Successful Tool for Genome Editing in Animal Models
Bilal Ahmed1, Mashaal Arif2, Muhammad Imran Qadir2
1School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu Province, PR China.
Abstract:
CRISPR/Cas9 is an innovative molecular tool that is utilized in advanced biological applications. This review focuses on modifying the genomes of a wide range of animals by CRISPR/Cas9 for greater usability and higher efficiency, providing an overview of the function and mechanism of this system and the utilization of this system for medicinal research. The type II CRISPR-Cas system is found in the Enterobacteriaceae bacteria family, which uses this system as a defense against invading phages and plasmids. This system can be engineered to direct its action toward a targeted site for the modification of a specific genome.
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