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Updated: Dec 13, 2025
08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
12.6K
In-Depth Analysis Reveals Production of Circular RNAs from Non-Coding Sequences
Annie Robic1, Julie Demars1, Christa Kühn2,3
1GenPhySE, Université de Toulouse, INRAE, ENVT, 31326 Castanet Tolosan, France.
Cells
|August 6, 2020
Summary
This study characterizes non-canonical circular RNAs (circRNAs), revealing most originate from non-coding sequences, not just back splicing. We identified novel circRNA types from intronic and mono-exonic non-coding genes.
Area of Science:
- Genomics
- RNA Biology
- Bioinformatics
Background:
- Circular RNAs (circRNAs) are crucial regulatory molecules.
- Total RNA sequencing after ribosomal depletion is standard for circRNA research.
- Non-canonical circRNAs, including those from non-coding genes, are less understood.
Purpose of the Study:
- To characterize non-canonical circRNAs, specifically those not from back splicing and those produced by non-coding genes.
- To investigate the origins and types of non-canonical circRNAs in porcine testis.
Main Methods:
- Analysis of total RNA sequencing data from porcine testis.
- Identification and classification of circRNAs based on junction reads and genomic origin.
- Focus on intron-derived and mono-exonic circRNAs.
Main Results:
- Canonical circRNAs have minimal contribution from long non-coding genes.
- Non-canonical circRNAs arise from intronic sequences (lariat-derived circRNAs, intron circles) and mono-exonic genes.
- A novel type, sub-exonic circRNAs, was identified from mono-exonic genes, including the ribozyme RNA RNase_MRP.
- Intronic circRNAs from ATXN2L suggest a potential new non-coding gene (stable intronic sequence RNA).
Conclusions:
- Most non-canonical circRNAs originate from non-coding sequences.
- Non-coding genes are a significant source of diverse circRNA structures.
- The study highlights the complexity of circRNA biogenesis beyond canonical back splicing.
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