Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Value operating system: a practical framework to measure outcomes-per-cost at the episode level.

BMJ open quality·2026
Same author

Directed evolution of compact RNA-guided nucleases for enhanced activity in mammalian cells.

Genome biology·2026
Same author

A modified Delphi consensus on tenosynovial giant cell tumour and giant cell tumour of bone : a report from the Birmingham Orthopaedic Oncology Meeting (BOOM).

The bone & joint journal·2026
Same author

Complications of PI to PIII hemipelvic resections for intermediate and malignant tumours : a systematic review and meta-analysis.

Bone & joint open·2026
Same author

Treatment effect heterogeneity of radiotherapy in localized Ewing sarcoma: A secondary analysis of the EURO-E.W.I.N.G. 99 and Ewing 2008 trial.

European journal of cancer (Oxford, England : 1990)·2026
Same author

Twelve-Month Metastatic State as a Landmark-Based Prognostic Stratifier in Metastatic Sarcoma.

Cancers·2026

Related Experiment Video

Updated: Dec 13, 2025

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.5K

Osteosarcoma-Derived Extracellular Vesicles Induce Lung Fibroblast Reprogramming.

Alekhya Mazumdar1,2, Joaquin Urdinez1,2, Aleksandar Boro1

  • 1Department of Orthopedics, Balgrist University Hospital, CH-8008 Zurich, Switzerland.

International Journal of Molecular Sciences
|August 6, 2020
PubMed
Summary
This summary is machine-generated.

Osteosarcoma (bone cancer) EVs activate lung fibroblasts, promoting cancer spread. Transforming growth factor beta 1 (TGFβ1) in these EVs drives this fibroblast activation, aiding pre-metastatic niche formation.

Keywords:
extracellular vesicleslung fibroblastsosteosarcomatumor microenvironmenttumor-host interactions

More Related Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.5K
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

6.1K

Related Experiment Videos

Last Updated: Dec 13, 2025

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.5K
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.5K
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

6.1K

Area of Science:

  • Oncology
  • Cell Biology
  • Cancer Metastasis Research

Background:

  • Tumor-secreted extracellular vesicles (EVs) mediate cancer-host communication.
  • Osteosarcoma frequently metastasizes to the lungs, but lung cell interactions are unclear.

Purpose of the Study:

  • To investigate if osteosarcoma EVs induce lung fibroblast differentiation.
  • To elucidate the mechanism by which osteosarcoma EVs modulate lung stromal cells.

Main Methods:

  • In vitro treatment of human lung fibroblasts with osteosarcoma EVs.
  • Analysis of fibroblast invasive competence, α-smooth muscle actin, and fibronectin expression.
  • Utilized transforming growth factor beta receptor 1 (TGFBR1) inhibitors and CRISPR-Cas9 gene editing.

Main Results:

  • Osteosarcoma EVs induced lung fibroblast differentiation into myofibroblasts/cancer-associated fibroblasts.
  • EV treatment increased fibroblast invasive capacity, α-smooth muscle actin, and fibronectin.
  • Transforming growth factor beta 1 (TGFβ1) within EVs was identified as the key driver of fibroblast activation.

Conclusions:

  • Osteosarcoma-derived EVs are novel regulators of lung fibroblast activation.
  • TGFβ1 in EVs plays a critical role in modulating lung fibroblasts for pre-metastatic niche formation.
  • Provides mechanistic insight into osteosarcoma lung metastasis.