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Updated: Dec 13, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Instrumentation-Free Semiquantitative Immunoanalysis Using a Specially Patterned Lateral Flow Assay Device.

Kyung Won Lee¹, Ye Chan Yu¹, Hyeong Jin Chun¹

¹Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea.

|August 6, 2020

Summary

This study introduces a novel semi-quantitative lateral flow immunoassay (SQ-LFI) that uses colored spots for naked-eye quantification. This innovation eliminates the need for external equipment, enabling easy estimation of analyte concentration in resource-limited settings.

Keywords:

instrument-free quantitative analysis lateral flow immunoassay microalbuminuria renal failure

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Last Updated: Dec 13, 2025

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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Area of Science:

Biomedical Engineering
Analytical Chemistry
Immunodiagnostics

Background:

Traditional colorimetric lateral flow immunoassays (LFAs) using gold nanoparticles (AuNPs) require external equipment for signal quantification.
The single test line in conventional LFAs presents a limitation for naked-eye analysis.
There is a need for simple, equipment-free diagnostic tools, especially in resource-limited environments.

Purpose of the Study:

To develop a semi-quantitative lateral flow immunoassay (SQ-LFI) system for naked-eye quantification of analytes.
To eliminate the need for external optical instruments in LFA signal detection.
To demonstrate the utility of the SQ-LFI system in practical immunoassays.

Main Methods:

Modified the conventional LFA test zone into patterned spots forming herringbone patterns.
Utilized AuNPs as optical probes in a sandwich immunoassay format.
Quantified analyte concentration by counting the number of colored spots, correlating with analyte concentration.

Main Results:

The number of colored spots in the SQ-LFI system increased proportionally with analyte concentration.
Successfully analyzed microalbumin in artificial urine samples, with a detection range of 0–500 μg/mL.
Achieved a limit of detection (LOD) of 15.5 μg/mL for microalbumin.

Conclusions:

The developed SQ-LFI system enables naked-eye semi-quantification of analytes without external equipment.
This system is applicable for practical immunoanalysis, as demonstrated with microalbumin detection.
The SQ-LFI offers a promising solution for efficient, low-cost diagnostic devices in resource-limited settings.