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Micro-Raman Spectroscopy Analysis of Optically Trapped Erythrocytes in Jaundice
Sanu Susan Jacob1, Aseefhali Bankapur2, Surekha Barkur2
1Department of Physiology, Kasturba Medical College-Manipal, Manipal Academy of Higher Education, Manipal, India.
Frontiers in Physiology
|August 6, 2020
Summary
Jaundice harms red blood cells (RBCs). Micro-Raman spectroscopy revealed distinct spectral markers in RBCs from jaundiced patients, offering insights into disease-related cellular stress and potential anemia exacerbation.
Area of Science:
- Biophysics
- Spectroscopy
- Hematology
Background:
- Jaundice involves bilirubin buildup, affecting red blood cells (RBCs).
- Circulating RBCs face toxic effects from bilirubin and inflammatory compounds in jaundice.
- Understanding RBC behavior under disease stress is crucial for managing conditions like anemia.
Purpose of the Study:
- To investigate the vibrational spectroscopy of live red blood cells in jaundice.
- To identify spectral markers of cellular stress in RBCs using micro-Raman spectroscopy.
- To explore the impact of disease-related stress milieus on RBC function.
Main Methods:
- Utilized micro-Raman spectroscopy combined with optical-trap technology.
- Optically immobilized live red blood cells from healthy volunteers and jaundiced patients.
- Probed RBCs using a 785 nm diode laser and analyzed spectral data with multivariate methods.
Main Results:
- Raman signatures from jaundiced RBCs showed significant variations compared to normal RBCs.
- Identified specific spectral markers indicative of cellular stress in jaundice.
- Demonstrated the sensitivity of Raman spectroscopy in detecting molecular changes in RBCs.
Conclusions:
- Micro-Raman spectroscopy can differentiate RBCs affected by jaundice.
- Identified spectral markers provide insights into RBC dysfunction and potential anemia exacerbation.
- Raman spectroscopy is a valuable tool for real-time, in situ cellular analysis in disease states.
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