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Tear Protein Analysis in Neonates Using High-Performance Liquid Chromatography-Light Emitting Diode Induced

Alisha Rizvi1,2, Sphurti S Adigal3, Sulatha V Bhandary2

  • 1Department of Ophthalmology, Hamdard Institute of Medical Sciences and Research and HAHC Hospital, New Delhi, India (Current Affiliation).

Korean Journal of Ophthalmology : KJO
|April 23, 2026
PubMed
Summary
This summary is machine-generated.

This study compared tear protein profiles in preterm and term neonates using HPLC-LED-IF. Differences in tear protein profiles were observed, suggesting potential biomarkers for neonatal development.

Keywords:
HPLC-LED-IFNeonatal tear protein analysisNon-invasivePrincipal component analysisTerm and pre-term

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Area of Science:

  • Neonatal research
  • Proteomics
  • Biomarker discovery

Background:

  • Neonatal intensive care units (NICUs) require advanced diagnostic tools.
  • Tear fluid analysis offers a non-invasive method for assessing neonatal health.
  • Understanding protein differences in neonatal tears can aid in developmental assessments.

Purpose of the Study:

  • To characterize and compare tear fluid protein profiles in preterm and term neonates.
  • To evaluate the efficacy of a cost-effective High-Performance Liquid Chromatography-Light Emitting Diode-Induced Fluorescence (HPLC-LED-IF) system for neonatal tear analysis.
  • To identify potential protein biomarkers differentiating neonatal groups.

Main Methods:

  • Collected unstimulated tear fluid samples from 40 term and 40 preterm neonates using Schirmer's strips.
  • Analyzed protein profiles using a HPLC-LED-IF system.
  • Applied Principal Component Analysis (PCA) to evaluate chromatographic data for group distinctions.

Main Results:

  • Observed differences in relative peak intensities and retention-time features between term and preterm neonates.
  • Exploratory PCA showed partial separation of groups, particularly in the 1620-1860 s retention window.
  • Demonstrated reproducibility in comparative profiling of low-volume neonatal tear samples.

Conclusions:

  • HPLC-LED-IF is a viable method for reproducible, comparative profiling of neonatal tear fluid.
  • Identified exploratory pattern-level distinctions in tear protein profiles between term and preterm neonates.
  • Further research with protein identification and larger cohorts is warranted to validate findings.