Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A bactericidal effect for human lactoferrin.

R R Arnold, M F Cole, J R McGhee

    Science (New York, N.Y.)
    |July 15, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mapping key features of transcriptional regulatory circuitry in embryonic stem cells.

    Cold Spring Harbor symposia on quantitative biology·2008
    Same author

    Effects of natural cross-linkers on the stability of dentin collagen and the inhibition of root caries in vitro.

    Caries research·2008
    Same author

    Measurement of TGF-beta in biological fluids.

    Current protocols in immunology·2008
    Same author

    Signaling cascades triggered by bacterial metabolic end products during reactivation of Kaposi's sarcoma-associated herpesvirus.

    Journal of virology·2007
    Same author

    Novel approaches for the induction of T helper 1 (Th1)- or Th2-type mucosal and parenteral immune responses.

    Expert opinion on investigational drugs·2005
    Same author

    Salmonella-mediated mucosal cell-mediated immunity.

    Cellular and molecular biology (Noisy-le-Grand, France)·2002
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    Human apolactoferrin effectively killed Streptococcus mutans and Vibrio cholerae, but not E. coli. Its metal-chelating ability is crucial for this bactericidal effect, reducing bacterial viability.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Immunology

    Background:

    • Lactoferrin is an iron-binding protein found in human secretions.
    • Its antimicrobial properties are well-documented, but specific mechanisms require further elucidation.

    Purpose of the Study:

    • To investigate the bactericidal activity of purified human apolactoferrin against specific bacterial species.
    • To determine the role of metal-chelating properties in lactoferrin's antimicrobial action.

    Main Methods:

    • Incubation of purified human apolactoferrin with Streptococcus mutans, Vibrio cholerae, and Escherichia coli.
    • Quantification of viable colony-forming units to assess bactericidal effects.
    • Evaluation of the impact of metal chelation on antimicrobial activity.

    Related Experiment Videos

    Main Results:

    • Apolactoferrin demonstrated significant bactericidal activity against Streptococcus mutans and Vibrio cholerae.
    • Escherichia coli exhibited resistance to the effects of apolactoferrin.
    • Sub-inhibitory concentrations of apolactoferrin markedly reduced bacterial viability.
    • The bactericidal effect was directly linked to the metal-chelating capacity of lactoferrin.

    Conclusions:

    • Purified human apolactoferrin possesses potent bactericidal properties against key pathogens like S. mutans and V. cholerae.
    • The metal-chelating function of lactoferrin is essential for its antimicrobial efficacy.
    • These findings highlight lactoferrin's potential as a therapeutic agent against susceptible bacterial infections.