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Updated: Dec 12, 2025

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
Published on: May 12, 2023
Investigating conformational changes of Prefoldin β1 as result of applying external mechanical force without any
Mohammad Askarian1, Reza Hasanzadeh Ghasemi2, Majid Moavenian1
1Department of Mechanical Engineering, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract:
Manipulating molecular scale bio-nanorobots and influencing their behaviour is one of the major challenges of new researches. Many coiled coil type proteins are involved in important biological functions due to physical properties that make them ideal for both nanoscale manipulation and sensing. The Prefoldin beta subunit from Thermococcus strain KS-1(Prefoldin β1) is one of the possible proteins that can serve as a new bio-nano-actuator. Besides having a balanced architecture, Prefoldin β1 can exhibit a wide range of exclusive authorities. In this study, steered molecular dynamics simulation is applied along with the centre of mass pulling and analyses of Prefoldin β1 conformational changes to characterise some of those abilities. Thus, applying external mechanical force without any position constraint shows that it has no movement throughout simulations. This proposes a novel method to capture different sizes and shapes of cargoes. During simulations, each arm was found to be very flexible, allowing it to enlarge its central cavity and capture different cargoes. For a more accurate analysis, the variations in the cavity of nano-actuator are investigated qualitatively and quantitatively with different parameters. Also, the force analysis of the arms can provide us with decent information about the performance of this nano-actuator.
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