Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vaccine Production01:23

Vaccine Production

144
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
144
Vaccines01:21

Vaccines

98
Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the...
98

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SARS-CoV-2 infection produces an IL-33-dependent chronic eosinophilic pneumonia and muco-inflammatory airways disease in mice.

JCI insight·2026
Same author

Finding the Goldilocks zone: Modulating glycoprotein cleavage and fusogenicity optimizes the efficacy of a candidate Crimean-Congo hemorrhagic fever virus vaccine.

bioRxiv : the preprint server for biology·2026
Same author

Microbiota-dependent interferon-λ controls immunity of the uterus and maternal-fetal interface.

Research square·2026
Same author

Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptase.

Nature structural & molecular biology·2026
Same author

Neutralizing antibodies elicited in nonhuman primates by an enterovirus D68 virus-like particle vaccine target receptor binding sites.

Science translational medicine·2026
Same author

Loss of nsp14-exonuclease activity impairs the replication, proofreading, fitness, and pathogenesis of SARS-CoV-2.

mBio·2026

Related Experiment Video

Updated: May 4, 2026

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
12:09

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

43.3K

SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness.

Kizzmekia S Corbett1, Darin K Edwards2, Sarah R Leist3

  • 1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Nature
|August 7, 2020
PubMed
Summary

A new mRNA vaccine, mRNA-1273, targets the SARS-CoV-2 spike protein. This vaccine generates strong immune responses and protects mice from COVID-19 infection without causing harm.

More Related Videos

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
10:58

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

7.9K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.9K

Related Experiment Videos

Last Updated: May 4, 2026

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
12:09

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

43.3K
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
10:58

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

7.9K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.9K

Area of Science:

  • Vaccinology
  • Virology
  • Immunology

Background:

  • The COVID-19 pandemic necessitates effective vaccines against SARS-CoV-2.
  • Stabilizing the prefusion state of Betacoronavirus spike proteins enhances immunogenicity.

Purpose of the Study:

  • To evaluate the immunogenicity and protective efficacy of mRNA-1273, an mRNA vaccine encoding a stabilized prefusion SARS-CoV-2 spike protein.

Main Methods:

  • Design of mRNA-1273 encoding a stabilized prefusion SARS-CoV-2 spike protein.
  • Assessment of neutralizing antibody and CD8+ T cell responses in mice.
  • Evaluation of protection against SARS-CoV-2 infection in mouse lungs and noses.

Main Results:

  • mRNA-1273 induced potent neutralizing antibody responses against wild-type and D614G mutant SARS-CoV-2.
  • The vaccine elicited CD8+ T cell responses.
  • Protection against SARS-CoV-2 infection was observed in mouse lungs and noses without immunopathology.

Conclusions:

  • mRNA-1273 demonstrates potential as a COVID-19 vaccine candidate.
  • The vaccine elicits robust humoral and cellular immunity.
  • Further clinical evaluation in phase III trials is warranted.