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RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

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Updated: Dec 12, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Analyzing editosome function in high-throughput.

Cristian Del Campo¹, Wolf-Matthias Leeder¹, Paul Reißig¹

¹Molecular Genetics, Technical University Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany.

Nucleic Acids Research

|August 7, 2020

Summary

This summary is machine-generated.

Researchers developed a new assay to study RNA editing in trypanosomes. This method aids in discovering drugs targeting these essential parasitic complexes.

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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

Area of Science:

Molecular Biology
Parasitology
Biochemistry

Background:

Mitochondrial gene expression in trypanosomatids relies on U-nucleotide specific RNA editing.
This essential process is catalyzed by the editosome, a complex unique to trypanosomatids.
The editosome is a promising drug target for treating trypanosome infections.

Purpose of the Study:

To develop an improved in vitro assay for monitoring editosome function.
To enable high-throughput screening for potential trypanocidal compounds.

Main Methods:

Utilized fluorophore-labeled substrate RNAs for RNA editing analysis.
Employed automated, high-throughput capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection.
Developed a multiplex fluorophore-labeling strategy for simultaneous U-insertion and U-deletion assays.

Main Results:

The developed assay is robust and suitable for high-throughput screening (HTS).
Requires only nanogram quantities of biological material.
Successfully scrutinizes both U-insertion and U-deletion editing reactions.

Conclusions:

The improved assay meets HTS performance criteria.
Facilitates the discovery of trypanosome-specific pharmaceuticals.
Offers a valuable tool for targeting essential parasitic RNA editing machinery.