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Interleukin 1 induces CD1 antigen expression on human gingival epithelial cells
1Department of Social and Preventive Dentistry, University of Queensland Dental School, Brisbane, Australia.
The Journal of Investigative Dermatology
|January 1, 1988
Summary
Interleukin-1 (IL-1) stimulates CD1 antigen expression on human Langerhans cells (LC). An IL-1 inhibitor blocks this effect, suggesting a combined role in regulating LC CD1 expression in gingival epithelium.
Area of Science:
- Immunology
- Cell Biology
- Dermatology
Background:
- CD1 antigen is a specific marker for human Langerhans cells (LC).
- Previous research showed Interleukin-1 (IL-1) and IL-1 inhibitor (ILS) influence CD1 expression on LC in organ culture.
Purpose of the Study:
- To investigate the effects of human IL-1 and ILS on CD1 expression in dispersed epithelial cell cultures.
- To determine the optimal dose of IL-1 for modulating CD1 expression.
- To understand the interplay between IL-1 and ILS in regulating CD1 expression on LC.
Main Methods:
- Utilized dispersed epithelial cell cultures.
- Applied organ-culture derived human IL-1, recombinant human IL-1, and purified ILS.
- Examined CD1 expression in both whole and CD1-depleted cell cultures.
Main Results:
- Both IL-1 preparations significantly stimulated CD1 expression at an optimal dose of 0.5 U/ml.
- Higher IL-1 concentrations did not further increase CD1 expression, indicating a finite responsive cell pool.
- The ILS effectively abrogated IL-1-induced CD1 expression in all tested cultures.
Conclusions:
- Human IL-1 directly induces CD1 expression on Langerhans cells in epithelial cultures.
- An IL-1 inhibitor counteracts IL-1's effect, highlighting a regulatory mechanism.
- IL-1 and its inhibitor collaboratively modulate CD1 expression on gingival Langerhans cells.