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OsRE1 interacts with OsRIP1 to regulate rice heading date by finely modulating Ehd1 expression
Juntao Chai1, Shanshan Zhu2, Chaonan Li2
1National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
Plant Biotechnology Journal
|August 7, 2020
Summary
Researchers identified OsRE1 and OsRIP1 as new regulators of Early heading date 1 (Ehd1) in rice. These factors fine-tune Ehd1 expression, impacting heading date and potentially improving rice adaptability.
Area of Science:
- Plant genetics
- Molecular biology
- Agronomy
Background:
- Heading date is crucial for rice yield.
- Early heading date 1 (Ehd1) is a key regulator of heading date in rice.
- Direct regulators of Ehd1 expression are not fully identified.
Purpose of the Study:
- Identify novel direct regulators of Ehd1.
- Characterize the function of OsRE1 and its interacting protein OsRIP1 in heading date determination.
- Investigate the cooperative mechanism of OsRE1 and OsRIP1 in modulating Ehd1 expression.
Main Methods:
- Promoter binding assays to identify OsRE1 interaction with Ehd1.
- Analysis of OsRE1 and OsRIP1 expression patterns.
- Genetic analysis of OsRE1 and OsRIP1 in regulating heading date.
- Phenotypic analysis of rice mutants.
Main Results:
- OsRE1 directly binds to the Ehd1 promoter, increasing Ehd1 expression and causing an early heading phenotype.
- OsRE1 is a nucleus-localized bZIP transcription factor with diurnal rhythmic expression.
- OsRIP1 interacts with OsRE1 and represses Ehd1 expression in an OsRE1-dependent manner.
- OsRE1 and OsRIP1 function upstream of Ehd1 to regulate heading date.
Conclusions:
- OsRE1 and OsRIP1 cooperatively regulate rice heading date by fine-tuning Ehd1 expression.
- These minor regulators offer potential for improving rice regional adaptability through precise heading date adjustments.
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