Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Dec 12, 2025

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

A guide to measuring phagosomal dynamics.

Roni Levin-Konigsberg¹, Adriana R Mantegazza^2,3

¹Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.

The FEBS Journal

|August 7, 2020

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

AI-Enhanced Adaptive Virtual Screening Platform Enabling Exploration of 69 Billion Molecules Discovers Structurally Validated FSP1 Inhibitors.

bioRxiv : the preprint server for biology·2026

Same author

The lysosomal carrier SLC29A3 supports antibacterial signaling, and promotes autophagy by activating TRPML1 in murine dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

RIG-I-dependent tumor-intrinsic type I interferon signaling restricts growth in breast cancer 3D culture.

bioRxiv : the preprint server for biology·2025

Same author

An SLC12A9-dependent ion transport mechanism maintains lysosomal osmolarity.

Developmental cell·2024

Same author

Drug Discovery in Low Data Regimes: Leveraging a Computational Pipeline for the Discovery of Novel SARS-CoV-2 Nsp14-MTase Inhibitors.

bioRxiv : the preprint server for biology·2023

Same author

SLC12A9 is a lysosome-detoxifying ammonium - chloride co-transporter.

bioRxiv : the preprint server for biology·2023

Phagocytosis involves phagosome maturation and resolution, crucial for immunity and homeostasis. This guide details assays measuring these dynamic cellular events for immune surveillance and adaptive responses.

Area of Science:

Cell Biology
Immunology

Background:

Phagocytosis is a key cellular process for immunity and homeostasis, executed by phagocytes.
Phagosome maturation involves dynamic fusion and fission events with the endolysosomal system.
Phagosome maturation links to antigen presentation and adaptive immunity.

Purpose of the Study:

To provide a guide on measuring phagosome maturation and resolution.
To elucidate the dynamic events and organelle crosstalk in phagosome lifecycle.

Main Methods:

Biochemical assays
Imaging techniques
Photoluminescence assays
Cytometric methods
Immune-based assays

Keywords:

antigen presentation autophagy dendritic cell inflammation macrophage membrane contact sites pattern recognition receptors phagocytosis phagosome maturation phagosome resolution

More Related Videos

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Published on: April 5, 2017

Study of Phagolysosome Biogenesis in Live Macrophages

Study of Phagolysosome Biogenesis in Live Macrophages

Published on: March 10, 2014

Related Experiment Videos

Last Updated: Dec 12, 2025

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Published on: April 5, 2017

Study of Phagolysosome Biogenesis in Live Macrophages

Study of Phagolysosome Biogenesis in Live Macrophages

Published on: March 10, 2014

Main Results:

Phagosome maturation involves interactions with the endolysosomal system.
Cargo nature influences phagosome integrity, potentially triggering inflammation and autophagy.
Phagosome resolution involves catabolite metabolism, phagosome resorption, and vesicle recycling.

Conclusions:

Phagosome maturation and resolution are dynamic, multi-step processes essential for phagocyte function.
Understanding these processes is vital for immune surveillance and maintaining tissue homeostasis.
Various assays are available to measure and analyze phagosome dynamics.