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Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions.
Rebecca M Dew1, Quinn S McFrederick2, Sandra M Rehan1
1Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
Insects
|August 8, 2020
Summary
Wild bees like the small carpenter bee (Ceratina calcarata) maintain consistent gut microbes across their range, regardless of local pollen or climate. This suggests conserved microbial communities are key to bee health.
Area of Science:
- Ecology
- Microbiology
- Entomology
- Pollinator Health
Background:
- Wild bees are crucial pollinators, obtaining microbes from their environment.
- Understanding wild bee diets and associated microbes is limited.
- Pollen provisions are vital for bee offspring development.
Purpose of the Study:
- To test if pollen and microbial composition in bee diets vary geographically.
- To investigate the floral resources and associated bacteria of the widespread bee Ceratina calcarata.
Main Methods:
- Amplicon sequencing of pollen provisions from Ceratina calcarata populations.
- Analysis of bacterial genera associated with pollen across different sites.
- Comparison of floral use and nesting substrate preferences.
Main Results:
- Ceratina calcarata utilized diverse floral resources across its range.
- Bacterial genera in pollen provisions were highly consistent across populations.
- Key bacteria included Acinetobacter, Erwinia, Lactobacillus, Sodalis, Sphingomonas, and Wolbachia.
Conclusions:
- Microbial communities in bee brood provisions are conserved within the species.
- Microbial composition is not primarily driven by climate or local pollen.
- Conserved microbes likely play a significant role in larval health and adaptation.
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