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Disorders of Leukocytes

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune...

Classification of Leukocytes

Classification of Leukocytes

Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm.
Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...

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Related Experiment Video

Updated: Dec 12, 2025

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

The Absolute Basophil Count.

Elena Borzova¹

¹Department of Dermatology and Venereology, I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation.

Methods in Molecular Biology (Clifton, N.J.)

|August 9, 2020

Summary

Accurate absolute basophil counts are crucial. Flow cytometry offers a precise, statistically superior method for enumerating basophils, surpassing limitations of manual and automated techniques.

Keywords:

Absolute basophil count Basopenia Basophilia Basophils Flow cytometry Hemocytometer Leukocyte differential Metachromatic staining Peripheral blood smear

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Last Updated: Dec 12, 2025

Basophil Activation Test for Allergy Diagnosis

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Published on: May 31, 2021

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Area of Science:

Hematology
Clinical Pathology
Immunology

Background:

Accurate absolute basophil counts are vital for diagnosing and monitoring various hematological and immunological conditions.
Current reference methods, like manual peripheral blood smear counting, have recognized limitations including distribution, observer, and statistical errors.
Automated hematology analyzers provide high throughput but exhibit inconsistent analytical performance for basophil enumeration.

Purpose of the Study:

To review and compare various techniques for determining the absolute basophil count in peripheral blood.
To highlight the potential of flow cytometry as a novel candidate reference method for absolute basophil counting.
To discuss emerging technologies for cell counting and their applicability to basophil enumeration.

Main Methods:

Manual counting of peripheral blood smears and cell counting chambers.
Automated hematology analyzers.
Flow cytometric enumeration using monoclonal antibodies, including double-platform, bead-based, and volumetric approaches.

Main Results:

Manual methods are the reference but suffer from significant limitations.
Automated analyzers offer speed but lack consistent accuracy for basophils.
Flow cytometry demonstrates high precision and statistical superiority for absolute basophil counting.

Conclusions:

Flow cytometry is a promising, highly precise candidate reference method for absolute basophil counts.
Further validation of emerging technologies like imaging flow cytometry and mass cytometry is needed.
Selecting the appropriate method depends on balancing accuracy, throughput, and available technology.