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Related Experiment Video

Updated: Dec 12, 2025

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Embryonic MTHFR contributes to blastocyst development.

Hiroki Ishitani1, Shuntaro Ikeda2, Kai Egashira1

  • 1Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.

Journal of Assisted Reproduction and Genetics
|August 9, 2020
PubMed
Summary

Embryonic methylenetetrahydrofolate reductase (MTHFR) is crucial for bovine blastocyst development. Knocking down MTHFR significantly impairs embryo quality and cell numbers, highlighting its essential role.

Keywords:
BlastocystFolate cycleMTHFRPreimplantation

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Methylenetetrahydrofolate reductase (MTHFR) genetic variations are controversially linked to human subfertility.
  • Direct evidence of embryonic MTHFR's role in mammalian preimplantation development is lacking.

Purpose of the Study:

  • To investigate the expression patterns of MTHFR during bovine preimplantation development.
  • To determine the functional significance of embryonic MTHFR in mammalian embryo development.

Main Methods:

  • MTHFR expression analyzed using RT-qPCR and public RNA-seq data.
  • MTHFR was knocked down in bovine embryos from the 8- to 16-cell stage using siRNA.
  • Effects of knockdown on preimplantation development were assessed.

Main Results:

  • MTHFR expression is high in oocytes, decreases in early cleavage stages, and recovers by the blastocyst stage.
  • MTHFR knockdown significantly reduced the blastocyst rate.
  • Knockdown led to decreased total, trophectoderm, and inner cell mass cell numbers.

Conclusions:

  • Embryonic MTHFR is essential for normal bovine blastocyst development.
  • Findings offer insights into MTHFR's role in fertility.
  • Results may inform strategies for improving embryo care through folate-related nutritional modulation.