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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Updated: Dec 12, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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MS Imaging-Guided Microproteomics for Spatial Omics on a Single Instrument.

Frédéric Dewez1,2, Janina Oejten3, Corinna Henkel3

  • 1Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Universiteitssingel 50, Maastricht, 6229 ER, The Netherlands.

Proteomics
|August 9, 2020
PubMed
Summary

Mass spectrometry imaging (MSI) combined with liquid chromatography-mass spectrometry (LC-MS) offers comprehensive molecular analysis. Dual-source mass spectrometry instruments enable both techniques on a single tissue section, preserving spatial information for detailed omics studies.

Keywords:
laser microdissectionmass spectrometry imagingmicroproteomicsspatial omics

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Area of Science:

  • Analytical Chemistry
  • Molecular Imaging
  • Biotechnology

Background:

  • Mass spectrometry imaging (MSI) provides spatial distribution of compounds in tissues but has limited analytical depth.
  • Coupling MSI with sensitive omics approaches is crucial for comprehensive molecular characterization while retaining spatial data.
  • Previous MSI-guided omics required separate, specialized mass spectrometry instruments.

Purpose of the Study:

  • To demonstrate the capability of a dual-source mass spectrometry instrument for integrated MSI and liquid chromatography-mass spectrometry (LC-MS) analysis.
  • To show that spatial information from MSI can be retained for subsequent LC-MS studies on the same tissue section.
  • To validate the use of dual-source instrumentation for sensitive proteomic analysis of local tissue extracts.

Main Methods:

  • Utilized a dual-source mass spectrometry instrument equipped with both matrix assisted laser/desorption ionization (MALDI) and electrospray ionization (ESI) sources.
  • Performed lipid-based MSI at high mass and high lateral resolution.
  • Conducted sensitive LC-MS analyses on local protein extracts from the exact same tissue section, guided by MSI data.

Main Results:

  • The dual-source instrument successfully performed both lipid MSI and LC-MS on a single tissue section.
  • High mass and high lateral resolution were achieved for lipid MSI.
  • Sensitive LC-MS analysis of local protein extracts was demonstrated, retaining spatial context.

Conclusions:

  • Dual-source mass spectrometry instruments can integrate MSI and LC-MS, enabling comprehensive molecular characterization of tissue sections.
  • This approach preserves spatial information, facilitating follow-up omics studies.
  • The technology promises advanced capabilities for analyzing the spatial distribution and composition of biomolecules within biological tissues.