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Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three-yellow chicken
Tao Sun1, Ximei Wang2, Wei Han1
1Technical Center of Qingdao Customs, NO. 70 Qutangxia Road, Qingdao, 266002, Shandong, China.
Abstract:
In this study, an avian leukosis virus (ALV) strain (GX-2020-01) was isolated from a three-yellow chicken, and its complete genome was 7570 bp long with the typical organization "5'LTR-gag-pol-env-3'LTR." Phylogenetic analysis and sequence comparison revealed that it belongs to the ALV-J subgroup. However, the LTR region of GX-2020-01 is highly similar to that of reference strains of ALV-K/E (96.61%-97.10%), demonstrating that this novel isolate is a natural recombinant. The replication efficiency of GX-2020-01 was significantly lower than the previously isolated ALV-J strain (NX0101), indicating that the recombination event might have resulted in slower virus replication, making it harder for it to be detected through routine testing.
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